Toxicity to aquatic algae and cyanobacteria.001 to 003

Toxicity to aquatic algae and cyanobacteria.001

Administrative data

Study result type:

read-across from supporting substance (structural analogue or surrogate)

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

Non-standardized test method, and insufficient documentation. Therefore concluded to be not reliable.

Data source

Reference

Reference Type:

publication

Title:

Toxicity of heavy metals and salts to Eurasian watermilfoil (Miriophyllum spicatum)

Author:

Stanley, R.A. and Arch, I.

Year:

1974

Bibliographic source:

Environ. Contam. Toxicol. 2(4):331-341.

Materials and methods

Test guideline

Qualifier:

according to

Principles of method if other than guideline:

Method: other: see freetext

GLP compliance:

no

Test materials

Identity of test material same as for substance defined in section 1 (if not read-across):

no

Test material identityopen allclose all

Test material identity 1

Test material identity 2

Test material identity 3

Analytical monitoring:

no

Test organisms

Test organisms (species):

other: Myrophilium spicatum (Eurasian watermilfoil)

Study design

Limit test:

no

Results and discussion

Effect concentrationsopen allclose all

Effect concentrations 1

Duration:

32 d

Endpoint:

EC50

Effect conc.:

10 228 mg/L

Basis for effect:

other: root weight

Effect concentrations 2

Duration:

32 d

Endpoint:

other: EC50 shoot length

Effect conc.:

4 120 mg/L

Basis for effect:

other: root weight

Effect concentrations 3

Duration:

32 d

Endpoint:

other: EC50 shoot weight

Effect conc.:

9 376 mg/L

Basis for effect:

other: root weight

Effect concentrations 4

Duration:

32 d

Endpoint:

other: EC50 root length

Effect conc.:

10 370 mg/L

Basis for effect:

other: root weight

Any other information on results incl. tables:

RESULTS: EXPOSED 
- Nominal/measured concentrations: nominal 
- Effect data/Element values: not described 
- Cell density data: not described 
- Growth curves: yes

Applicant's summary and conclusion

*********************************************************************

Toxicity to aquatic algae and cyanobacteria.002

Administrative data

Study result type:

read-across from supporting substance (structural analogue or surrogate)

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

Reference not translated.

Data source

Reference

Reference Type:

publication

Title:

Institut Agrochimiceskich Problem [Effect of high quantities of magnesium, potassium and sodium on the growth and biochemical indices of chlorella.]

Author:

Oganesyan, D.A., Mikaelyan K.A., Barseghyan M.N.

Year:

1977

Bibliographic source:

Gidroponiki Hagordoimner 16:26-31.

Materials and methods

Test guideline

Qualifier:

according to

Principles of method if other than guideline:

Method: other

Test materials

Identity of test material same as for substance defined in section 1 (if not read-across):

no

Test material identityopen allclose all

Test material identity 1

Test material identity 2

Test material identity 3

Test organisms

Test organisms (species):

Chlorella pyrenoidosa

Results and discussion

Effect concentrations

Duration:

8 d

Endpoint:

EC100

Effect conc.:

57 700 mg/L

Applicant's summary and conclusion

Conclusions:

Reference in foreign language and not translated.

Executive summary:

Considered unreliable not readable.

Validity criteria fulfilled:

no

I

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Toxicity to aquatic algae and cyanobacteria.003

Administrative data

Purpose flag:

key study

Study result type:

read-across from supporting substance (structural analogue or surrogate)

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

Study is missing too much information to be able to assign reliability score. However due to the very low toxicity of sodium sulfate in all ecotoxicological endpoints and the comparable reference substance data, data from this study may be used with care. Previous expert opinion has also recognised the limitations of the study but permitted its use see below for iuclid 4 / SIDS rationale. 
Iuclid 4 Rationale:
In this test a different algae species is used than
recommended in the OECD-guidelines. When the results of
Potassium dichromate (72 h, EbC50) used as a reference 
substance, are compared with the results given in the 
EC-directive 92/69/EEC (mean 0.53 mg/l, range 0.20 - 0.75
mg/l) the sensitivity of Nitzschia linearis is not
significantly different from that of Selenastrum
capricornutum or Scenedesmus subspicatus.

It is recognised that the duration of this study was greater
than the recommended OECD study time but as the reference
result from this study is on the 
lower boundary of the EC recommendation, the two results are considered comparable. 

This study would be considered as validity 3 because of the
shortcomings. However, based on the other data, Sodium sulfate is a substance of very 
low toxicity and the results
of this study confirm this, therefore this study is
evaluated as valid with restrictions (2).

Data source

Reference

Reference Type:

publication

Title:

The relative sensitivity of diatoms, snails, and fish to twenty common constituents of industrial wastes.

Author:

Patrick, R.J., Cairns, J., Scheier, A.

Year:

1968

Bibliographic source:

Fish-Cult. 30(3):137-140

Materials and methods

Test guideline

Qualifier:

according to

Principles of method if other than guideline:

Pre-Guideline study was intended to look at variation between different species for common industrial wastes.

GLP compliance:

no

Remarks:

not applicable: pre-GLP

Test materials

Identity of test material same as for substance defined in section 1 (if not read-across):

no

Test material identityopen allclose all

Test material identity 1

Test material identity 2

Test material identity 3

Details on test material:

No specific information on the test material was given. All chemicals were of American Chemical Society grade.
Sodium Sulfate

Analytical monitoring:

no

Test organisms

Test organisms (species):

other: Nitzschia linearis

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

120 h

Remarks:

Static

Results and discussion

Effect concentrations

Duration:

120 h

Endpoint:

EC50

Effect conc.:

1 900 mg/L

Any other information on results incl. tables:

Iuclid 4 Data

- Nominal/measured concentrations: nominal 
- Effect data/Element values: growth (cell counts) 
- Cell density data: not described 
- Growth curves: not described 
- Reference substance: Potassium dichromate, TLm = 0.208 mg/l

Applicant's summary and conclusion

Conclusions:

This result shows that Sodium sulfate is not a substance of concern for diatoms. The reference test data are comparable to that of data for OECD 
approved algae species. The response of the tested diatom species is therefore likely to be similar to approved test species. The data may therefore be used with care.

Validity criteria fulfilled:

no