Short-term toxicity to aquatic invertebrates.006 to 008

Short-term toxicity to aquatic invertebrates.006

Administrative data

Study result type:

read-across from supporting substance (structural analogue or surrogate)

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

Lacking to much information to be considered reliable.

Data source

Reference

Reference Type:

publication

Title:

Acute toxicity of heavy metals (copper, lead, zinc), phenol and sodium on Allium cepa l., Lepidium sativum l. and Daphnia magna St.: comparitive investigations and the practical applications.

Author:

Arambasic, M.B., Bjelic, S., Subakov, G.

Year:

1995

Bibliographic source:

Water Res. 29(2):497-503.

Materials and methods

Principles of method if other than guideline:

Method: other: see freetext

GLP compliance:

no

Test materials

Identity of test material same as for substance defined in section 1 (if not read-across):

no

Test material identityopen allclose all

Test material identity 1

Test material identity 2

Test material identity 3

Details on test material:

IUCLID4 Test substance: as prescribed by 1.1 - 1.4

Analytical monitoring:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Limit test:

no

Results and discussion

Effect concentrations

Duration:

48 h

Endpoint:

EC50

Effect conc.:

9 124 mg/L

Any other information on results incl. tables:

RESULTS: EXPOSED 
- Nominal/measured concentrations: nominal 
- Effect data (Immobilisation): not described 
- Concentration / response curve: not described 
- Cumulative immobilisation: not described 
- Effect concentration vs. test substance solubility: not 
described  
- Other effects: not described

Applicant's summary and conclusion

Conclusions:

Lacking too much critical information

***********************************************************************************

Short-term toxicity to aquatic invertebrates.007

Administrative data

Purpose flag:

supporting study

Study result type:

read-across from supporting substance (structural analogue or surrogate)

Study period:

not specified

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

Lacking Analysis ,GLP

Data source

Reference

Reference Type:

publication

Title:

Toxicity of laundry detergent components to a freshwater cladoceran and their contribution to detergent toxicity

Author:

Warne, J.

Year:

1999

Bibliographic source:

Ecotoxicology and environmental Safety 44,196-206 (1999)

Materials and methods

Principles of method if other than guideline:

The acute 48-h immobilization tests were conducted using methods developed by the NSW Environment Protection Protection Authority (Warne and Julli, in press)

Test materials

Identity of test material same as for substance defined in section 1 (if not read-across):

no

Test material identityopen allclose all

Test material identity 1

Test material identity 2

Test material identity 3

Details on test material:

Sodium Sulphate anhydrous CAS( 7757-82-6) and Sodium sulphate decahydrate CAS ( 7727-73-3). No detailed information on source or purity of 
test chemicals was given.

Analytical monitoring:

no

Vehicle:

no

Test organisms

Test organisms (species):

Ceriodaphnia dubia

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Results and discussion

Effect concentrationsopen allclose all

Effect concentrations 1

Duration:

48 h

Endpoint:

EC50

Effect conc.:

6 129.96 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks (e.g. 95% CL):

Decahydrate

Effect concentrations 2

Duration:

48 h

Endpoint:

EC50

Effect conc.:

3 150.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks (e.g. 95% CL):

anhydrous

Any other information on results incl. tables:

Results showed that the above components were not of concern with regards to there contribution to detergent toxicity.

Applicant's summary and conclusion

Conclusions:

This study may be considered reliable as supporting evidence only.

Executive summary:

Guideline followed, methodology sound, not GLP , no analysis and lacking test substance ID data.

**************************************************************************

Short-term toxicity to aquatic invertebrates.008

Administrative data

Purpose flag:

supporting study

Study result type:

read-across from supporting substance (structural analogue or surrogate)

Study period:

not specified

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

Test was conducted to standard EPA guidelines with analysis. Lacking GLP and some detail expected of a modern report . Can be considered reliable however. Iuclid 4 rationale: Test performed according to standardized EPA guideline for testing of effluents, with determination of test concentrations. Test parameters pH and 
oxygen measured but not all reported. No information about controls. Replicate test performance.

Data source

Reference

Reference Type:

publication

Title:

Statistical models to predict the toxicity of major ions to Ceriodaphnia dubia, Daphnia magna and Pimephales promelas

Author:

Mount, D.R., Gulley, D.D., Hockett, J.R., Garrison, T.D.,Evans, J.M.

Year:

1997

Bibliographic source:

Environmental Toxicology and Chemistry, Vol. 16, No. 10, pp. 2009-2019

Materials and methods

Principles of method if other than guideline:

Method: other: see freetext, based on EPA/600/4-90/027 (1991) guideline

GLP compliance:

no

Remarks:

Research project on modelling

Test materials

Identity of test material same as for substance defined in section 1 (if not read-across):

no

Test material identityopen allclose all

Test material identity 1

Test material identity 2

Test material identity 3

Details on test material:

Test substances were all of reagent grade or higher supplied by Sigma Aldrich

Sodium Sulphate

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

Test solutions were prepared by dissolving 10,000 mg/l of the individual ion salts in moderately hard reconstituted water.

Test organisms

Test organisms (species):

Ceriodaphnia dubia

Details on test organisms:

All organisms used in testing were obtained from in-house cultures (ENSR, Fort Collins, CO. USA); daphnids were less than 24 h old at test initiation.
Ceriodaphnia dubia were cultured in either moderately hard reconstituted water (MHRW) or 20% mineral water at 25ºC. D. magna were cultured in 
hard reconstituted water 20°C.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

moderately hard reconstituted water (MHRW)

Test temperature:

25 ºC

pH:

between pH 7.5 and 9.0

Dissolved oxygen:

Measured DO concentrations were always within an acceptable range (>40% saturation)

Nominal and measured concentrations:

Due to this study being a summary of many different tests and test substances nominal and measured values per test substance was not reported.

Details on test conditions:

All tests were conducted in 30-ml plastic beakers containing 10 ml of test solution and five organisms per chamber. 
Tests were conducted under a 16h : 8h light : dark photoperiod

Reference substance (positive control):

yes

Remarks:

NaCl

Results and discussion

Effect concentrationsopen allclose all

Effect concentrations 1

Effect concentrations 2

Details on results:

Result suggest Sodium sulphate is not toxic to Ceriodaphnia species

Results with reference substance:

LC50 values were calculated for the reference toxicant tests from the first 11 test group. The variance calculated was used in the model to 
compensate for possible variation in the culture.

Reported statistics and error estimates:

STATISTICAL METHODS: logistic multiple regression.

Any other information on results incl. tables:

The results in the publication are given as mg sulfate ion, these values were converted to mg sodium sulfate.

Applicant's summary and conclusion

Conclusions:

Study was carried out according to EPA guidelines and was reported in sufficient detail to be considered reliable with restrictions. It can be concluded that sodium sulphate is not a substance of concern for Ceriodaphnia dubia.

Executive summary:

Lacking some detail expected of a substance specific report. Not conducted to GLP and certificate of analysis was not included. However this paper can be considered further reliable. Methods were reported clearly, guidelines were followed and analytical support for test substance conformation was carried out.

Validity criteria fulfilled:

yes